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h 1200 wash buffer  (Vector Laboratories)


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    Vector Laboratories h 1200 wash buffer
    H 1200 Wash Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mounting+buffer+with+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm41894395-234-35-32
    Average 98 stars, based on 21944 article reviews
    h 1200 wash buffer - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis
    Article Snippet: .. Following application of TrueView autofluorescence quencher, slides were mounted using Vectashield Vibrance Antifade mounting media with DAPI (SP-8500-15; Vector Laboratories; USA) and air dried for 2 h before imaging on the Nikon Ti2-E microscope. ..

    Microscopy:

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis
    Article Snippet: .. Following application of TrueView autofluorescence quencher, slides were mounted using Vectashield Vibrance Antifade mounting media with DAPI (SP-8500-15; Vector Laboratories; USA) and air dried for 2 h before imaging on the Nikon Ti2-E microscope. ..

    Recombinant:

    Article Title: A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion.
    Article Snippet: Catalog # H-003-62 c-Fos Antibody (4) Santa Cruz Biotechnology, Inc. Cat# sc-52, RRID:AB_2106783 Alexa Fluor® 594 AffiniPureTM Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 711-585-152, RRID: AB_2340621 Alexa Fluor® 647 AffiniPureTM Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 711-605-152, RRID: AB_2492288 Living Colors® mCherry Monoclonal Antibody Clontech Laboratories, Inc. Cat # 632543 acetylated α Tubulin (6-11B-1) Santa Cruz Biotechnology, Inc. Cat# sc-23950 Alexa Fluor® 594 AffiniPureTM Donkey Anti-Mouse IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 715-585-150 RRID: AB_2340854 Guinea pig anti-insulin Agilent (Dako) Cat# A0564, RRID:AB_10013624 Somatostatin Antibody (G-10) Santa Cruz Biotechnology, Inc. Cat# sc-55565 Jo urn al Pr e-p roo f Rabbit anti-Rabies virus Glycoprotein G polyclonal Antibody MyBioSource Catalog # MBS1491332 Recombinant Anti-TVA antibody [PICA187E] abcam Cat# ab271292 Alexa Fluor® 594 AffiniPureTM Donkey Anti-Rat IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 712-585-153 RRID: AB_2340689 Bacterial and Virus Strains RabV CVS-N2c-deltaG-EGFP-EnvA Gift from Matthias J. Schnell, Thomas Jefferson University N/A pAAV-EF1a-double floxed- hChR2(H134R)-mCherry-WPRE- HGHpA Addgene Catalog # 20297-AAV8 pAAV-hSyn-DIO-hM3D(Gq)- mCherry Addgene Catalog # 44361-AAV8 pAAV-hSyn-DIO-mCherry Addgene Catalog # 50459-AAV8 AAV-DJ CMV DIO eGFP-2A-TeNT Gene Vector and Virus Core Stanford University Cat# GVVC-AAV-71 Chemicals, Peptides, and Recombinant Proteins Clozapine UN2811-III (6.1) MP Biomedicals Item # 0215976010 Critical Commercial Assays Mouse Ultrasensitive Insulin ELISA Jumbo ALPCO Diagnostics Catalog# 80-INSMSU-E10 Jo urn al Pr e-p roo f Mouse C-Peptide ELISA Kit MyBioSource Catalog# MBS761428 Mouse Acetylcholine ELISA Kit abcam Catalog # ab287812 Mouse Acetylcholine ELISA Kit MyBioSource Catalog # MBS8807517 RNAqueousTM-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 NEBNext® UltraTM II RNA Library Prep Kit for Illumina® NEB Cat# E7770S HD-XG Gluc, Temp, Act, mouse 1.5 cm, SQ Data Sciences International (DSI) Cat# 270-0194-003 RNAscope® HiPlex kit Advanced Cell Diagnostics, Inc. N/A Deposited Data RNA sequencing data GEO GSE281091 Data S1 N/A Excel file containing values used to create all graphs in the paper. .. Experimental Models: Organisms/Strains C57BL/6J The Jackson Laboratory Strain #:000664 RRID:IMSR_JAX:000664 B6(Cg)-Ins1tm1.1(cre)Thor/J (Ins1cre) The Jackson Laboratory Strain #:026801 RRID:IMSR_JAX:026801 B6;129P2-Gt(ROSA)26Sortm1(CAG- RABVgp4,-TVA)Arenk/J (RphiGT, RΦGT) The Jackson Laboratory Strain #:024708 RRID:IMSR_JAX:024708 B6.Cg-Gt(ROSA)26Sortm6(CAGZsGreen1)Hze/J (Ai6 or Ai6(RCL- ZsGreen) The Jackson Laboratory Strain #:007906 RRID:IMSR_JAX:007906 B6;129S-Cartpttm1.1(cre)Hze/J ((Cart- IRES2-Cre-D) The Jackson Laboratory Strain #:028533 RRID:IMSR_JAX:028533 Jo urn al Pr e-p roo f jGCaMP8s Gift from Krashes Lab N/A Oligonucleotides Recombinant DNA Software and Algorithms Olympus OlyVIA 3.2.1 Olympus https://www.olympus-sis.com/ Ponemah v6.30 DSI https://www.datasci.com/ Partek Flow (Build version 11.0.24.0102) Partek https://www.partek.com/ RNAscopeTM HiPlex Image Registration Software v2.0 Advanced Cell Diagnostics, Bio-Techne Brand https://acdbio.com/ ImageJ/ Fiji National Institutes of Health https://imagej.net/ij/ GraphPad Prism v10.1.0 (316) GraphPad Software, LLC https://www.graphpad.com/ Adobe Photoshop Version: 25.4.0 Adobe Inc. https://www.adobe.com/ Adobe Illustrator Version: 28.2 Adobe Inc. https://www.adobe.com/ Cellpose3 bioRxiv https://doi.org/10.1101/2024.02.10.57 9780 Others VECTASHIELD® Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Meloxicam SR ZooPharm N/A Jo urn al Pr -pr oo f Normal Donkey Serum Jackson ImmunoResear ch Laboratories, Inc. Code: 017-000-121 RRID: AB_2337258 Artificial cerebrospinal fluid (ACSF) Bio-Techne R&D Systems Catalog #: 3525 Bio-ServTM Dustless Precision PelletsTM Grain-Based Rodent Diet Bio-ServTM F0163 Catalog# 14-726-581 Microvette® CB 300 EDTA K2E SARSTEDT Order number: 16.444.100 C&B-Metabond® Quick! .. Luting Cement Patterson Dental Patterson Item # 553-3484 Dental Cement Kit, 1 lb, Pink Opaque StoeltingTM Item # 51458

    Software:

    Article Title: A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion.
    Article Snippet: Catalog # H-003-62 c-Fos Antibody (4) Santa Cruz Biotechnology, Inc. Cat# sc-52, RRID:AB_2106783 Alexa Fluor® 594 AffiniPureTM Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 711-585-152, RRID: AB_2340621 Alexa Fluor® 647 AffiniPureTM Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 711-605-152, RRID: AB_2492288 Living Colors® mCherry Monoclonal Antibody Clontech Laboratories, Inc. Cat # 632543 acetylated α Tubulin (6-11B-1) Santa Cruz Biotechnology, Inc. Cat# sc-23950 Alexa Fluor® 594 AffiniPureTM Donkey Anti-Mouse IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 715-585-150 RRID: AB_2340854 Guinea pig anti-insulin Agilent (Dako) Cat# A0564, RRID:AB_10013624 Somatostatin Antibody (G-10) Santa Cruz Biotechnology, Inc. Cat# sc-55565 Jo urn al Pr e-p roo f Rabbit anti-Rabies virus Glycoprotein G polyclonal Antibody MyBioSource Catalog # MBS1491332 Recombinant Anti-TVA antibody [PICA187E] abcam Cat# ab271292 Alexa Fluor® 594 AffiniPureTM Donkey Anti-Rat IgG (H+L) Jackson ImmunoResear ch Laboratories, Inc. Code: 712-585-153 RRID: AB_2340689 Bacterial and Virus Strains RabV CVS-N2c-deltaG-EGFP-EnvA Gift from Matthias J. Schnell, Thomas Jefferson University N/A pAAV-EF1a-double floxed- hChR2(H134R)-mCherry-WPRE- HGHpA Addgene Catalog # 20297-AAV8 pAAV-hSyn-DIO-hM3D(Gq)- mCherry Addgene Catalog # 44361-AAV8 pAAV-hSyn-DIO-mCherry Addgene Catalog # 50459-AAV8 AAV-DJ CMV DIO eGFP-2A-TeNT Gene Vector and Virus Core Stanford University Cat# GVVC-AAV-71 Chemicals, Peptides, and Recombinant Proteins Clozapine UN2811-III (6.1) MP Biomedicals Item # 0215976010 Critical Commercial Assays Mouse Ultrasensitive Insulin ELISA Jumbo ALPCO Diagnostics Catalog# 80-INSMSU-E10 Jo urn al Pr e-p roo f Mouse C-Peptide ELISA Kit MyBioSource Catalog# MBS761428 Mouse Acetylcholine ELISA Kit abcam Catalog # ab287812 Mouse Acetylcholine ELISA Kit MyBioSource Catalog # MBS8807517 RNAqueousTM-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 NEBNext® UltraTM II RNA Library Prep Kit for Illumina® NEB Cat# E7770S HD-XG Gluc, Temp, Act, mouse 1.5 cm, SQ Data Sciences International (DSI) Cat# 270-0194-003 RNAscope® HiPlex kit Advanced Cell Diagnostics, Inc. N/A Deposited Data RNA sequencing data GEO GSE281091 Data S1 N/A Excel file containing values used to create all graphs in the paper. .. Experimental Models: Organisms/Strains C57BL/6J The Jackson Laboratory Strain #:000664 RRID:IMSR_JAX:000664 B6(Cg)-Ins1tm1.1(cre)Thor/J (Ins1cre) The Jackson Laboratory Strain #:026801 RRID:IMSR_JAX:026801 B6;129P2-Gt(ROSA)26Sortm1(CAG- RABVgp4,-TVA)Arenk/J (RphiGT, RΦGT) The Jackson Laboratory Strain #:024708 RRID:IMSR_JAX:024708 B6.Cg-Gt(ROSA)26Sortm6(CAGZsGreen1)Hze/J (Ai6 or Ai6(RCL- ZsGreen) The Jackson Laboratory Strain #:007906 RRID:IMSR_JAX:007906 B6;129S-Cartpttm1.1(cre)Hze/J ((Cart- IRES2-Cre-D) The Jackson Laboratory Strain #:028533 RRID:IMSR_JAX:028533 Jo urn al Pr e-p roo f jGCaMP8s Gift from Krashes Lab N/A Oligonucleotides Recombinant DNA Software and Algorithms Olympus OlyVIA 3.2.1 Olympus https://www.olympus-sis.com/ Ponemah v6.30 DSI https://www.datasci.com/ Partek Flow (Build version 11.0.24.0102) Partek https://www.partek.com/ RNAscopeTM HiPlex Image Registration Software v2.0 Advanced Cell Diagnostics, Bio-Techne Brand https://acdbio.com/ ImageJ/ Fiji National Institutes of Health https://imagej.net/ij/ GraphPad Prism v10.1.0 (316) GraphPad Software, LLC https://www.graphpad.com/ Adobe Photoshop Version: 25.4.0 Adobe Inc. https://www.adobe.com/ Adobe Illustrator Version: 28.2 Adobe Inc. https://www.adobe.com/ Cellpose3 bioRxiv https://doi.org/10.1101/2024.02.10.57 9780 Others VECTASHIELD® Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Meloxicam SR ZooPharm N/A Jo urn al Pr -pr oo f Normal Donkey Serum Jackson ImmunoResear ch Laboratories, Inc. Code: 017-000-121 RRID: AB_2337258 Artificial cerebrospinal fluid (ACSF) Bio-Techne R&D Systems Catalog #: 3525 Bio-ServTM Dustless Precision PelletsTM Grain-Based Rodent Diet Bio-ServTM F0163 Catalog# 14-726-581 Microvette® CB 300 EDTA K2E SARSTEDT Order number: 16.444.100 C&B-Metabond® Quick! .. Luting Cement Patterson Dental Patterson Item # 553-3484 Dental Cement Kit, 1 lb, Pink Opaque StoeltingTM Item # 51458

    Staining:

    Article Title: Targeting Nuclear Factor-κB1 With Andrographolide ― Novel Strategy to Inhibit Vascular Lesions and Aneurysm Formation in Abdominal Aortic Aneurysm ―
    Article Snippet: After the overnight incubation, samples were washed three times with PBS (5 min each) and then incubated with secondary antibodies (Alexa Fluor 488/594/647-conjugated IgG secondary antibodies; 1 : 200 dilution; A-11008/A-11001/A-11012/A-11032; Invitrogen) for 60 min at room temperature. .. After washing with PBS, samples were covered with Antifade Mounting Medium with DAPI stain solution (H-1200-10; Vector Laboratories, USA) and incubated for 5 min at room temperature in the dark to visualize nuclei. .. Two slides (6 sections each) per group were assessed under a Leica SP8 confocal microscope.

    Incubation:

    Article Title: Targeting Nuclear Factor-κB1 With Andrographolide ― Novel Strategy to Inhibit Vascular Lesions and Aneurysm Formation in Abdominal Aortic Aneurysm ―
    Article Snippet: After the overnight incubation, samples were washed three times with PBS (5 min each) and then incubated with secondary antibodies (Alexa Fluor 488/594/647-conjugated IgG secondary antibodies; 1 : 200 dilution; A-11008/A-11001/A-11012/A-11032; Invitrogen) for 60 min at room temperature. .. After washing with PBS, samples were covered with Antifade Mounting Medium with DAPI stain solution (H-1200-10; Vector Laboratories, USA) and incubated for 5 min at room temperature in the dark to visualize nuclei. .. Two slides (6 sections each) per group were assessed under a Leica SP8 confocal microscope.



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